Popular treatment of recent times; What is an Exosome?

Exosomes are bioactive substances that play a role in multiple biological and cellular activities of the skin. These nano-sized small membrane vesicles (30-100nm) are secreted by all eukaryotic cells, including skin cells. Exosomes, which were once thought to be a method of removing cellular wastes, are seen as important intercellular communication tools with current studies. Exosomes contain proteins such as mRNA, miRNA, cytokines and growth factors, and lipids that originate from host cells and direct various cellular responses in target cells. Therefore, we can benefit from these small vesicles whenever we want tissue repair, healing and regeneration.

How is Exosome Obtained?

Exosomes are small communication vesicles located outside of all nucleated cells. So there are different ways to get it.

The first of these is the most widely used exosomes of mesenchymal origin. Mesenchymal stem cells (MSCs) are multipotent cells with immunomodulatory and trophic effects. They can be collected from human adipose tissue. Adipose-derived stem cells (ADSCs) have regenerative abilities that promote proliferation, migration and secretory activity of keratinocytes and fibroblasts. Recent studies have shown that most of the beneficial effects of ADSCs can be achieved using cell-free extracts containing
extracellular vesicles such as cytokines and exosomes. The use of exosomes for therapeutic
applications depends on cell culture techniques, in which 3-D cultures are preferred. Isolation
techniques such as ultracentrifugation, ultrafiltration, and precipitation are required to produce high-quality exosomes suitable for commercial use. Exosomes are not immunogenic. They can also be loaded with drugs, making them valuable as drug delivery systems. Therefore, exosomes have enormous
potential as therapeutic and cosmetic components.

MSC exosomes can be produced in the genetics laboratory as a result of fat removal from the
person by the lipoaspirate method. Or ready-made mesenchymal exosome preparations can be used. Since exosomes do not contain cells, there is no risk of immunogenicity and tumorigenicity.
Another method is the PRP-exosome. Here exosomes are collected and produced from
platelets. Blood is taken from the person for the same PRP as it is filled and exosomes are
obtained in cell culture in the genetics laboratory.

In Which Indications Are Exosomes Used On The Skin?

Skin rejuvenation / Anti-aging
Spot treatments
acne scar treatments
Porous skin tightening
hair loss
Atopic dermatitis / Dermatitis

How Often Are Exosomes Administered?

Exosome treatment can be applied between 1 and 3 sessions depending on the condition of the
person’s skin.

How is Exosome Treatment Applied?

In order to increase the effectiveness of exosome treatment, treatment protocols are
created in our clinic. It is usually applied in combination with laser or microneedling treatments.